Essential equipment for all modules
- Phase contrast microscope with x20 x40 phase objective and x100 oil immersion (bright field)
- Heated microscope stage
- Positive displacement pipette (to pipette a minimum 50μl)
- Air displacement pipettes covering volumes up 1ml
- Centrifuge
- Vortex mixer
- Counter (bench-top multichannel mechanical or electronic)
- Haemocytometers (improved Neubauer)
- Humidified chamber (container with lid containing wet tissue)
Essential Consumables for all modules
- Dilution tubes (up to 1mL) for sperm concentration
- Glass slides (frosted end)
- 22x22mm cover slips (wet preparation and motility)
- 22x50mm cover slips (morphology)
- Sperm diluent (*WHO, 1999)
Additional Equipment for Module 3 “Sperm Cryopreservation”
- Protective cabinet (class II or laminar flow hood)
- Cryopreservation equipment - Controlled rate freezer, dry shipper or vapour phase dewar
Additional Consumables for Module 3 “Sperm Cryopreservation”
- Conical tubes for sperm preparation
- Sperm wash/resuspension media
- Sperm density gradient media 100% stock bottle
- Cryoprotectant
- Cryopreservation plastics (straws or vials)